Journal: bioRxiv

Article Title: Functionally selective dopamine D1 receptor endocytosis and signaling by catechol and non-catechol agonists

doi: 10.1101/2024.04.15.589637

Figure Lengend Snippet: HEK293 cells were transfected with HA-D1R and β-arrestin2-GFP. The cells were then treated with 3 µM of the indicated agonist and fixed after 10 minutes. The D1R was then detected using antibodies against the HA tag and visualized with alexa594 secondary antibodies. TIRF microscopy was used to image a thin slice of the cells at the plasma membrane. Punctate GFP signal indicates β-arrestin recruitment to the plasma membrane. A) Vehicle treatment did not recruit β-arrestin to the plasma membrane. B) SKF-81297 strongly recruited β-arrestin to the plasma membrane. β-arrestin colocalized in puncta with HA-D1R. C) A-77636 treatment induced β-arrestin recruitment to the plasma membrane that colocalized with the HA-D1R. D) PF-1119 weakly recruited β-arrestin to the plasma membrane. E) Cmpd 41 moderately recruited β-arrestin to the plasma membrane. F) Cmpd 19 strongly recruited β-arrestin to the plasma membrane and colocalized with HA-D1R. Similar results observed in >21 cells across three independent experiments. White boxes indicate the area that is enlarged in the corner of merged images. Scale bar = 10 μm.

Article Snippet: TIRF microscopy was performed using a Nikon Eclipse Ti2 dual epi fluorescence and confocal microscope outfitted with a TIRF imaging apparatus.

Techniques: Transfection, Microscopy, Clinical Proteomics, Membrane

Journal: The Journal of experimental biology

Article Title: Structural and functional characterization of the contractile aorta and associated hemocytes of the mosquito Anopheles gambiae .

doi: 10.1242/jeb.181107

Figure Lengend Snippet: Fig. 2. The location of the aorta in the head, neck and prothorax. (A) A mosquito head with the ventral side removed, exposing the pharyngeal plate (pp). (B) Resected pp with the aorta and alimentary tract attached. (C) A resected aorta (phalloidin; green) attached to the pp. (D) A resected aorta showing its position relative to the proboscis and alimentary tract, and its attachment to the pp; gc, gastric caeca; mp, maxillary palp. (E–G) In the thorax, the aorta lies just dorsal of the midgut, the crop and the location where the gc diverge from the midgut. The specimen in E is magnified in F (lateral view) and G (dorsal view). (H) The corpora allata lie alongside the aorta. Specimens were imaged under brightfield (monochrome) and/or fluorescence illumination (phalloidin, green; Hoechst 33342, blue). A, anterior; P, posterior; L, lateral; D, dorsal; V, ventral. Scale bars: A–G, 100 µm; H, 50 µm.

Article Snippet: Acquisition of still images Specimens were visualized under brightfield and/or epi-fluorescence illumination using a Nikon 90i compound microscope (Nikon Corporation, Tokyo, Japan) equipped with a Nikon Intensilight CHGFI fluorescence illumination unit.

Techniques: Fluorescence

Journal: The Journal of experimental biology

Article Title: Structural and functional characterization of the contractile aorta and associated hemocytes of the mosquito Anopheles gambiae .

doi: 10.1242/jeb.181107

Figure Lengend Snippet: Fig. 3. The aorta is composed of three regions: the anterior aorta, the bulbous chamber and the posterior aorta. (A) The entire aorta showing the arrangement of muscle fibers (phalloidin, green) and cardiomyocyte nuclei (Hoechst 33342, blue). Four high-resolution images were stitched to assemble this image, and portions (dotted squares) are magnified in B–F. am, alary muscle. (B,C) The excurrent opening (B) and musculature (C) of the anterior aorta (AA). (D,E) The anterior (D) and posterior (E) regions of the bulbous chamber (BC), including the cluster of nuclei at the posterior. (F) The posterior aorta (PA). Specimens were imaged under fluorescence illumination (phalloidin, green; Hoechst 33342, blue).

Article Snippet: Acquisition of still images Specimens were visualized under brightfield and/or epi-fluorescence illumination using a Nikon 90i compound microscope (Nikon Corporation, Tokyo, Japan) equipped with a Nikon Intensilight CHGFI fluorescence illumination unit.

Techniques: Fluorescence